The unicellular marine organism Salpingoeca rosetta belongs to the group of choanoflagellates. When trying to find micro organism, these protozoa can detect their prey by way of sure signaling molecules. They then type rosette-like multicellular buildings to higher catch their prey.

Nevertheless, some micro organism produce inhibitors to forestall the formation of the rosette form and thus shield themselves in opposition to the predators.

Chia-Chi Peng is among the two first authors of the paper. Supply: Anna Schroll/Leibniz-HKI

Scientists from the Leibniz-HKI Analysis Unit Chemical Biology of Microbe-Host Interactions have now chemically synthesized this bacterial inhibitor, the sulfonolipid IOR-1A. The brief, modular synthesis route additionally allowed them to provide functionalized derivatives, the impact of which on thechanoflagellate S. rosetta was additionally assessed.

The experiments have been performed in collaboration with the Molecular and Utilized Microbiology and the Biosynthetic Design of Pure Merchandise models of the Leibniz-HKI, in addition to with the College of California at Berkeley, USA.

The research have allowed “elaboration of the biochemical foundations of rosette-formation within the choanoflagellate S. rosetta,” the authors write within the journal Angewandte Chemie. In additional steps, they wish to examine the precise position of the detected goal proteins in cell differentiation of S. rosetta with the intention to decipher the mobile signaling pathways.

Choanoflagellates are thought of the closest residing unicellular family members of in the present day’s animals and have turn out to be an vital mannequin organism to review the evolution of multicellularity.

Mild and fluorescence micrograph of choanoflagellates. The fluorescence picture exhibits the uptake of the sulfonolipid (blue). Supply: Chia-Chi Peng/Leibniz-HKI

Unique publication

Raguž L, Peng CC, Rutaganira FUN, Krüger T, Stanišić A, Jautzus T, Kries H, Kniemeyer O, Brakhage AA, King N, Beemelmanns C (2022). Complete Synthesis and Practical Analysis of IORs, Sulfonolipid-based Inhibitors of Cell Differentiationin Salpingoeca rosetta. Angew. Chem. Int. Ed. 2022, e202209105

Written by Ronja Münch

Supply: Leibniz-HKI




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